Ziziphi iiThubhu zokuqokelelwa kwegazi ze-EDTA kwaye zisetyenziswa njani?

Kuvavanyo lwezonyango kunye nokuxilongwa kweklinikhi kunye nonyango,Iityhubhu zokuqokelela igazi ze-EDTA, njengezinto ezisetyenziswayo eziphambili zokuqokelela igazi, zidlala indima ebalulekileyo ekuqinisekiseni ukunyaniseka kweesampuli kunye nokuchaneka kovavanyo. Kweli nqaku, siya kuhlalutya ngokubanzi lo "mgcini ongabonakaliyo" kwintsimi yezonyango ukusuka kwimiba yenkcazo, ukuhlelwa kombala, umgaqo we-anticoagulation, injongo yokuvavanya kunye nokusetyenziswa komgangatho.

Yintoni iEDTA ityhubhu yokuqokelela igazi?

Ityhubhu ye-EDTA yokuqokelela igazi luhlobo lwetyhubhu yokuqokelela igazi ene-Ethylene Diamine Tetraacetic Acid okanye ityuwa yayo, esetyenziselwa ikakhulu ukuqokelela iisampuli zegazi kunye nonyango lwe-anticoagulant. I-EDTA inokuthintela i-coagulation cascade reaction ngokuchela i-calcium ion egazini, ukuze kugcinwe igazi likwimo yolwelo ixesha elide, kwaye ibonelele ngeesampulu ezizinzileyo zovavanyo lwendlela yegazi kunye nebhayoloji yemolekyuli. Ibonelela ngeesampulu ezizinzileyo zesiqhelo segazi, ibhayoloji yemolekyuli kunye nolunye uvavanyo.

Njengenxalenye ebalulekileyo yeizinto ezityiwayo zonyango, Iityhubhu zokuqokelela igazi ze-EDTA kufuneka zihambelane nomgangatho wesizwe "we-Single-ukusetyenziswa kwe-venous blood sample collection containers" (umz. GB/T 19489-2008) ukuqinisekisa ukusebenza kobunyumba, i-non-pyrogenic kunye ne-non-cytotoxicity.

Imibala eyahlukeneyo yeetyhubhu zokuqokelela igazi ze-EDTA

Ngokwemigangatho eqhelekileyo yamazwe ngamazwe (njengezikhokelo ze-CLSI H3-A6), iityhubhu zokuqokelela igazi ze-EDTA zihlala zigqunywe ngemfusa (EDTA-K2/K3) okanye eluhlaza okwesibhakabhaka (i-sodium citrate exutywe ne-EDTA) ukwahlula ukusetyenziswa:

Qaphela: Ezinye iibhrendi zinokukhowudwa ngeminye imibala, jonga imiyalelo phambi kokusetyenziswa.

I-Anticoagulation mechanism ye-EDTA yokuqokelela igazi

I-EDTA ngeqela layo le-molecular carboxyl (-COOH) kunye ne-calcium ions egazini (Ca²⁺) idityaniswe ukuze yenze i-chelate ezinzileyo, ngaloo ndlela inqanda ukusebenza kwe-plasminogen, ivala inkqubo yokudibanisa i-fibrinogen kwi-fibrin. Le anticoagulation inezi mpawu zilandelayo:

1. Ukuqala ngokukhawuleza kwesenzo: i-anticoagulation ingagqitywa kwimizuzu eyi-1-2 emva kokuqokelela igazi;

2. ukuzinza okuphezulu: iisampuli zingagcinwa ngaphezu kweeyure ze-48 (ifriji inokwandiswa kwiiyure ze-72);

3. Uluhlu olubanzi lwesicelo: lufanelekile kwiimvavanyo ezininzi ze-hematology, kodwa kungekhona kwi-coagulation okanye iimvavanyo zeplatelet function (i-sodium citrate tubes iyadingeka).

Izinto eziphambili zovavanyo lwetyhubhu yokuqokelela igazi ye-EDTA

1. Uhlalutyo lwegazi oluqhelekileyo: inani leeseli ezimhlophe zegazi, iiparamitha zeseli ebomvu, ukugxininiswa kwe-hemoglobin, njl;

2. ukuchongwa kweqela legazi kunye nokudibanisa: Iqela legazi le-ABO, ukufumanisa i-Rh factor;

3. ukuxilongwa kwemolekyuli: uvavanyo lwemfuzo, ukumiselwa komthamo wentsholongwane egazini (umz. HIV, HBV);

4. i-glycated hemoglobin (HbA1c): ukujonga ixesha elide le-glucose yegazi kwi-diabetes mellitus;

5. ukuhlolwa kwe-parasite yegazi: I-Plasmodium, ukubonwa kwe-microfilariae.

Ukusetyenziswa kwemimiselo kunye nezilumkiso

1. Inkqubo yokuqokelela:

Emva kokubulala iintsholongwane eluswini, sebenza ngokomgangatho wokuqokelela igazi le-venous;

Ngokukhawuleza emva kokuqokelela, guqula ityhubhu yokuqokelela igazi ngamaxesha angama-5-8 ukuqinisekisa ukuba i-anticoagulant ixutywe ngokupheleleyo negazi;

Gwema ukugubha ngamandla (ukuthintela i-hemolysis).

2. Ugcino kunye nothutho:

Gcina kwindawo yokushisa (15-25 ° C), ugweme ukushisa okanye ukukhenkceza;

Beka ngokuthe nkqo ngexesha lokuthutha ukukhusela ukukhulula umnqwazi wetyhubhu.

3. iimeko zokuchasana:

Iityhubhu ze-sodium citrate ziyafuneka kwi-Coagulation IV (PT, APTT, njl.);

Uvavanyo lomsebenzi wePlatelet lufuna ityhubhu ye-sodium citrate.

Indlela yokukhetha umgangatho ophezuluEDTA ityhubhu yokuqokelela igazi?

1. Ukufaneleka kunye nesiqinisekiso: khetha iimveliso eziphumelele i-ISO13485 kunye ne-CE certification. 2;

2. Ukhuseleko lwezinto eziphathekayo: umzimba wombhobho kufuneka ube sobala kwaye ungabikho intsalela yeplastiki;

3. I-dosing echanekileyo: inani le-anticoagulant elongezelelweyo kufuneka lihambelane nomgangatho wesizwe (umz. I-EDTA-K2 igxininise ye-1.8± 0.15mg / mL);

4. Udumo lweBrand: I-priority inikwa ii-brand ezaziwayo-kakuhle kwinkalo yezinto ezisetyenziswayo zonyango ukuqinisekisa ukuzinza kwebhetshi.

Ukuqukumbela

Njengelungu eliphambili leisixhobo sokuqokelela igazi, Iityhubhu zokuqokelela igazi ze-EDTA zinempembelelo ngokuthe ngqo ekuchanekeni kweziphumo zovavanyo ngokweempawu zabo ze-anticoagulant. Ngokumisela umgangatho wokusetyenziswa kwemibhobho yokuqokelela igazi enemibala eyahlukeneyo kwaye idibanise neenkqubo ezingqongqo zokuqokelela, inokubonelela ngesiseko esithembekileyo sokuxilongwa kweklinikhi. Kwixesha elizayo, ngokuphuhliswa kweyeza ezichanekileyo, iibhubhu zokuqokelela igazi ze-EDTA ziya kudlala indima ebaluleke ngakumbi kuhlalutyo lwegazi, ukulandelelana kwemfuza kunye nezinye iinkalo, kwaye ziqhubeke nokukhusela impilo yabantu.